Part:BBa_K4799502:Design
pNtNRAMP2
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 952
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 952
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 952
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 952
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 952
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
promoter function test
In our biotechnology experimental design, we plan to initially attempt connecting our promoter pNtNRAMP2 separately with two different functional genes, GUS and GFP on two different carriers, to test whether our promoter can initiate the expression of these genes when the plant is exposed to a cadmium environment. We used transient infiltration of Nicotiana benthamina leaves to minimize the duration required for obtaining performance of pNtNRAMP2.
If feasible, we will then integrate it into the luminescent reporter gene. We chose pNtNRAMP2 based on literature research, please see the listed reference below.
Source
Nicotiana tabacum
References
[1] K. Kozak, A. Papierniak-Wygladala, M. Palusińska, A. Barabasz, and D. M. Antosiewicz, ‘Regulation and Function of Metal Uptake Transporter NtNRAMP3 in Tobacco’, Front. Plant Sci., vol. 13, p. 867967, May 2022, doi: 10.3389/fpls.2022.867967. [2] R.-T. Li et al., ‘MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter’, Int. J. Mol. Sci., vol. 24, no. 14, p. 11278, Jul. 2023, doi: 10.3390/ijms241411278. [3] Y. Zhang et al., ‘The Expression of the StNRAMP2 Gene Determined the Accumulation of Cadmium in Different Tissues of Potato’, Int. J. Mol. Sci., vol. 24, no. 11, p. 9322, May 2023, doi: 10.3390/ijms24119322.
[4] I. Ullah, Y. Wang, D. J. Eide, and J. M. Dunwell, ‘Evolution, and functional analysis of Natural Resistance-Associated Macrophage Proteins (NRAMPs) from Theobroma cacao and their role in cadmium accumulation’, Sci. Rep., vol. 8, p. 14412, Sep. 2018, doi: 10.1038/s41598-018-32819-y.